journal of men's health
Volume 6, Issue 1 , Pages 50-55 , March 2009

Flow cytometric measurement of sperm nuclear DNA fragmentation in infertile men with normal standard sperm parameters

  • Tzvetan H. Lukanov, MD, PhD

      Affiliations

    • Medical Center ‘Clinical Institute for Reproductive Medicine’, Pleven, and Center for Reproductive Health, Medical University of Pleven 1, Pleven, Bulgaria
    • Corresponding Author InformationCorresponding author.
    • ,
  • Danail I. Lichev, MD

      Affiliations

    • Medical Center ‘Clinical Institute for Reproductive Medicine’, Pleven, and Center for Reproductive Health, Medical University of Pleven, Pleven, Bulgaria
    • ,
  • Emiliana I. Konova, MD, PhD

      Affiliations

    • Medical Center ‘Clinical Institute for Reproductive Medicine’, Pleven, and Center for Reproductive Health, Medical University of Pleven, Pleven, Bulgaria
    • ,
  • Alkan I. Emin, MD

      Affiliations

    • Medical Center ‘Clinical Institute for Reproductive Medicine’, Pleven, and Center for Reproductive Health, Medical University of Pleven, Pleven, Bulgaria
    • ,
  • Nina P. Ayvazova

      Affiliations

    • Medical Center ‘Clinical Institute for Reproductive Medicine’, Pleven, Bulgaria
    • ,
  • Anjelika V. Velkova, MD, PhD

      Affiliations

    • Department of Social Medicine, Medical University of Pleven, Pleven, Bulgaria
    • ,
  • Roumen G. Roussev, MD, PhD

      Affiliations

    • Medical Center ‘Clinical Institute for Reproductive Medicine’, Pleven, Bulgaria and Millenova Immunology Laboratories, Chicago, Illinois, USA

Received 11 June 2008 ,Accepted 11 August 2008.

References 

  1. World Health Organization . Laboratory Manual for the Examination of Human Semen and Sperm–Cervical Mucus Interaction. 4th edition. Cambridge, UK: Cambridge University Press; 1999;
  2. Rowe PJ, Comhaire FH, Hargreave TB, Mellows HJ. WHO Manual for the Standardized Investigation and Diagnosis of the Infertile Couple. Cambridge, UK: Cambridge University Press; 1993;
  3. Holt WV, Van Look KJW. Concepts in sperm heterogeneity, sperm selection and sperm competition as biological foundations for laboratory tests of semen quality. Reproduction. 2004;127:527–535
  4. Nieschlag E. Scope and goal of andrology. In:  Nieschlag E,  Behre HM editor. Andrology: Male Reproductive Health and Dysfunction. Berlin: Springer; 2000;p. 1–8
  5. Evenson DP, Jost LK, Marshall D, Zinaman MJ, Clegg E, Purvis K, et al. Utility of the sperm chromatin structure assay as a diagnostic and prognostic tool in the human fertility clinic. Hum Reprod. 1999;14:1039–1049
  6. Evenson DP, Jost LK. Sperm chromatin structure assay is useful for fertility assessment. Methods Cell Sci. 2000;22:169–189
  7. Coulam CB, Ahlering P, Rodriquez H, Keskintepe L, Sher G, Roussev R. Pregnancy outcome after IVF/ET among couples who have elevated DNA fragmentation determined by the sperm DNA integrity assay (SDIa). Fert Steril. 2003;80(Suppl. 3):207;(Abstract)
  8. Ahlering P, Keskintepe L, Sher G, Fisch JD, Danner L, Maasarani G. The sperm DNA integrity assay (SDI(TM)) a valuable method for assessing sperm vitality in preparation for in vitro fertilization (IVF). Fert Steril. 2003;80(Suppl 3):113;(Abstract)
  9. Host E, Lindenberg S, Smidt-Jensen S. DNA strand breaks in human spermatozoa: correlation with fertilization in vitro in oligozoospermic men and in men with unexplained infertility. Acta Obstet Gynecol Scand. 2000;79:189–193
  10. Irvine DS, Twigg JP, Gordon EL, Fulton N, Milne PA, Aitken RJ. DNA integrity in human spermatozoa: relationships with semen quality. J Androl. 2000;21:33–44
  11. Trisini AT, Singh NP, Duty SM, Hauser R. Relationship between human semen parameters and deoxyribonucleic acid damage assessed by the neutral comet assay. Fertil Steril. 2004;82:1623–1632
  12. Cordelli E, Eleuteri P, Leter G, Rescia M, Spanò M. Flow cytometry applications in the evaluation of sperm quality: semen analysis, sperm function and DNA integrity. Contraception. 2005;72:273–279
  13. Spano M, Bonde JP, Hjollund HI, Kolstad HA, Cordelli E, Leter G. Sperm chromatin damage impairs human fertility. The Danish First Pregnancy Planner Study Team. Fertil Steril. 2000;73:43–50
  14. Larson KL, De Jonge CJ, Barnes AM, Jost LK, Evenson DP. Sperm chromatin structure assay parameters as predictors of failed pregnancy following assisted reproductive techniques. Hum Reprod. 2000;15:1717–1722
  15. Larson-Cook KL, Brannian JD, Hansen KA, Kasperson KM, Aarnold ET, Evenson DP. Relationship between the outcomes of assisted reproductive techniques and sperm DNA fragmentation as measured by the sperm chromatin structure assay. Fertil Steril. 2003;80:895–902
  16. Saleh RA, Agarwal A, Nada EA, El-Tonsy MH, Sharma RK, Meyer A, et al. Negative effects of increased sperm DNA damage in relation to seminal oxidative stress in men with idiopathic and male factor infertility. Fertil Steril. 2003;79:1597–1605
  17. Roussev RG, Coulam CB. Elevated sperm DNA fragmentation is associated with reproductive failure. Am J Reprod Immunol. 2004;51:472
  18. Sakkas D, Mariethoz E, Manicardi G, Bizzaro D, Bianchi P, Bianchi U. Origin of DNA damage in ejaculated human spermatozoa. Rev Reprod. 1999;4:31
  19. Saleh RA, Agarwal A, Nelson DR, Nada EA, El Tonsy MH, Alvares JG, et al. Increased sperm nuclear DNA damage in normozoospermic infertile men: a prospective study. Fertil Steril. 2002;78:313–318
  20. Bungum M, Humaidan P, Spano M, Jepson K, Bungum L, Giwercman A. The predictive value of sperm chromatin structure assay (SCSA) parameters for the outcome of intrauterine insemination, IVF and ICSI. Hum Reprod. 2004;19:1401–1408
  21. Lopes S, Sun J, Jurisicova A, Meriano J, Casper RF. Semen deoxyribonucleic acid fragmentation is increased in poor quality semen samples and correlates with failed fertilization in intracytoplasmic sperm injection. Fertil Steril. 1998;69:528–532
  22. Zini A, Libman J. Sperm DNA damage: clinical significance in the era of assisted reproduction. CMAJ. 2006;175:495–500
  23. O’Brien J, Zini A. Sperm DNA integrity and male infertility. Urology. 2005;65:16–22
  24. Aitken RJ, Krausz C. Oxidative stress, DNA damage and the Y chromosome. Reproduction. 2001;122:497–506
  25. Barroso G, Morshedi M, Oehninger S. Analysis of DNA fragmentation, plasma membrane translocation of phosphatidylserine and oxidative stress in human spermatozoa. Hum Reprod. 2000;15:1338–1344
  26. Zini A, Bielecki R, Phang D, Zenzes MT. Correlations between two markers of sperm DNA integrity, DNA denaturation and DNA fragmentation, in fertile and infertile men. Fertil Steril. 2001;75:674–677
  27. Giwercman A, Richthoff J, Hjollund H, Bonde JP. Correlation between sperm motility and sperm chromatin structure assay parameters. Fertil Steril. 2003;80:1404–1412
  28. Zini A, Kamal K, Phang D, Willis J, Jarvi K. Biologic variability of sperm DNA denaturation in infertile men. Urology. 2001;58:258–261

PII: S1875-6867(08)00154-1

doi: 10.1016/j.jomh.2008.08.002

journal of men's health
Volume 6, Issue 1 , Pages 50-55 , March 2009

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